Regulatory
Part:BBa_K4303031
Designed by: Arya Ajit Gohad Group: iGEM22_ICT-Mumbai (2022-12-16)
5'UTR
This part is an RBS- UTR Hybrid with a lower expression than the RBS itself. This was tested in E. coli of two strains: DH5alpha and BL21(DE3), and the protein expressed was RFP. The comparative performance in the strain DH5alpha was 0.1963 compared to the control (RBS only), with a standard deviation of 0.0111. The comparative performance in the strain BL21(DE3) was 0.2703 compared to the control (RBS only), with a standard deviation of 0.0163.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |